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Biotechnology Research Paper Topics & Free Essay Examples

GREEN FLUORESCENT PROTEIN (GFP)

GREEN FLUORESCENT PROTEIN ( GFP ) MUTANTS WITH ALTERED FLUORESCENCE INTENSITY AND EMMISSION SPECTRA Introduction: Now-a-days GFP is making revolution in the field of scientific discipline by its applications and properties.GFP is a stable protein extracted from the exposure variety meats of the jellyfish Aequoria Victoria by Shimomura et Al in 1962. In 1992 the…

GENETIC TRANSFORMATION OF INDICA RICE BPT5204 WITH CRY1Aa3 GENE

Introduction It is indispensable that we better our nutrient production and distribution in order to feed and maintain a turning universe population free from hungriness, while at the same clip cut downing the environmental impacts of high populations and bettering the quality life for most worlds. This will necessitate continued development and responsible use of…

Ames Test

The Spot-Overlay Ames Test was used in the lab to find the mutagenesis of Diet Coke and ThermaFlu. Along with these substances three mutant strains of salmonella were also tested. TA 1535, TA 1537, TA 1538 all lacked the ability to grow the amino acid histidine unless reverted back by the potential mutagens. After the…

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The Lambda Protocol Physics Investigation

This experiment is designed to produce ? -DNA bound in gel to fragment according to the restriction enzyme used to cut it up and to the sizes of these subsequent fragments. The aim of this experiment is to produce a -DNA fragmentation, which will be of known sizes because the entirety of the -DNA genome…

Biotechnology in Environment Clean Up Process

In the past decade or two, there has been a significant increase in the levels of environmental pollution mostly due to direct or indirect human activities. The major sources of environmental pollution are -Industries, Agricultural sources (mainly rural area), anta reporting SOUrces (man related activities mainly in urban areas), boogieing sources etc. The pollutants are…

Biochemistry Problems and Solutions

Because the rise per residue in an a helix is 1. K the length is 477 A (318*1. 5). (b) Eighteen residues in each strand (40 minus 4 divided by 2) are in a ; sheet conformation. Because the rise per residue is the length is 63 A. 2. Contrasting isomers . Poly-L-Lucien in an…

Genetically Modified Organisms

What are GMOS? GMO is the abbreviation for (Genetically modified organism) also known as GEO (genetically engineered organism), which is an organism whose genetic DNA has been altered using genetic engineering techniques. Techniques used are know to be recombinant DNA technology which uses the DNA molecules from different sources, which are combined to generate a…

Lab Report Banana Dna

DNA is organized into genes and stores genetic information. DNA molecules are long, slender molecules that carry the heritable information of organisms on to future generations. Because of their size, it is impossible to see a single DNA molecule with the naked eye. It would take about 300,000 DNA molecules side by side to make…

Gene Therapy: Lab Report

Sequence, so it could not replicate on its own and needed to be integrated by homologous recombination. The TRIP gene was a circular plasmid that contained an EARS, which allowed for it to act as an extra chromosome in the gene. The objective was to insert a “wild gene” and replace the defective genes and…

Karyotyping in Genetic Counseling Programme

Genetic counselling is a counselling process that deals with disorders within the family. This process involves a counsellor who helps the family or the person in the following ways: (a) discusses medical facts and informs about the diagnosis and the cause of the disorder and the cure; (b) informs the way heredity can cause disorders…

DNA and Genetic Privacy

The Merriam-Webster’s Collegiate Dictionary emphasizes that privacy is “freedom from unauthorized intrusion” (Dictionary). Genetic privacy is just that, something to be kept a personal affair and kept from illicit incursion.One’s genetic information should be kept out of the hands of the insurance agencies and, in special cases, law enforcement. A gene is hereditary material located…

Chapter 9 Dna-Based Information Technologies

Chapter 9 DNA-Based Information Technologies Multiple Choice Questions 1. DNA cloning: the basics Page: 307Difficulty: 1Ans: C Restriction enzymes: A) act at the membrane to restrict the passage of certain molecules into the cell. B) are highly specialized ribonucleases that degrade mRNA soon after its synthesis. C) are sequence-specific DNA endonucleases. D) are very specific…

Analysis Of Vntr (Variable Number Tandem Repeats) Or Str (Short Tandem Repeats) Loci Has Been Used In The Past To Establish Paternity In Cases Where The Biological Father Of A Child Is In Question. Below Are The Dna Fingerprints Of 5 People In Such As Case: The Child (C), The Mother (M), And Three Potential Fathers Of The Child (F1, F2, F3). In This Experiment, The Total Dna Of Each Individual Was Cut With A Restriction Enzyme Ran On Three Gels, And Southern Blotted. Each Blot Was Probed With A Radioactive Probe To Different Vntr Loci (A, B, And C) And The Results Are Shown Below. M C F1 F2 F3 M C F1 F2 F3 M C F1 F2 F3 Vntr A Probe Vntr B Probe Vntr C Probe Which Of These Is The Most Likely

DNA profiling DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals on the basis of their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a person’s DNA makeup, which can also be used as the…

Sleep

Sleep is a global state, the control mechanisms of which are manifested at every level of biological organization, from genes and intracellular mechanisms to networks of cell populations, and to all central neuronal systems at the organismic level, including those that control movement, arousal, autonomic functions, behavior and cognition.Recent genetic findings indicate that the molecular…

Biotechnology: Biological Weapons/Bioterrorism Essay Example

Biotechnology: Biological Weapons/Bioterrorism Essay Introduction Nowadays we are living in a world where progress is very much appreciated. Technology made our lives a whole lot easier and there are many inventions who had been a great help to us, mankind. However, does all this inventions promote the goods of all mankind? Or are these simply…

Genetic Modified Food Essay Example

Genetic Modified Food Essay Introduction Introduction Food is any material consumed to provide nutritional support for the body which it is probably from plant or animal that holds important nutrients, such as carbohydrates, proteins, vitamins, or minerals (Shah, 2006). Despite the importance of the food but it will be harmful in human health if it…

Parasites are organisms which benefit from living in or on a host

Parasites are organisms which benefit from living in or on a host organism at its expense. They vary greatly in size and appearance, ranging from single-celled microscopic protozoans, able to cause diseases such as malaria and “sleeping sickness”, to helminths like the tapeworm or lung fluke. The devastating effects of parasitic diseases are felt globally…

Chloe LeonardPHI105Christal AbronJuly 7 2019Stem cell therapy

Chloe Leonard PHI-105 Christal Abron July 7, 2019 Stem cell therapy can cause a breakthrough in the medical field providing Regenerative medicine, regenerate a stem cell pancreas for diabetics and hope for a new start in curing some possible diseases. There have been studies showing how well steam cell therapy are treating people well in…

ABSTRACTTransport of the drug through skin is best route of

ABSTRACT Transport of the drug through skin is best route of drug delivery because the skin is largest organ in human body. Drug carries which are used in transdermal drug delivery such as liposomes, niosomes, or microemulsions pose a problem that they remains mostly confined to the skin surface and therefore do not transport drugs…

Taken together all the progress in understanding the

Taken together, all the progress in understanding the molecular mechanisms facilitating neural lineages’ specification from hPSCs, provides a promising strategy for DNT predictions in vitro. The human CNS is considered one of the tissues most vulnerable to perturbations, since it consists of an ensemble of sophisticated biological processes (e.g. proliferation, migration, differentiation, apoptosis, neurite outgrowth,…

Assignment no 1ReviewPaperBIO732–Gene Manipulation and

Assignment no. 1 ReviewPaper BIO732–Gene Manipulation and Genetic Engineering Student Name: Muhammad Usman Student ID: ms170400902 Applications of recombinant DNA technology ABSTRACT: There are four essential uses of rDNA in the territories of human illnesses prophylaxis, treatment, conclusion and disclosure. Regions of prophylaxis incorporate antibodies and coagulation. It is currently conceivable, through rDNA innovation, to…

Literature Survey

? Literature Survey Bioinformatics By: Name: Yuvarani k Reg no:20172022061 Dept: Mca 2nd year ? Literature Survey Application Of Generic Algorithm Bioinformatics Abstract: Bioinformatics is the field we deal with biology, computer science and mathematics and it is useful to analyze the statics data, and it is very effective tool for understanding biological application like…

INTRODUCTIONCharacterized with an autosomal dominant pattern

INTRODUCTION Characterized with an autosomal dominant pattern of inheritance and complete penetrance, Achondroplasia is the most common, non-lethal chondrodysplasia with an estimated incidence of 2.6:100,000; with particularly higher rates noted in Denmark and Latin America. Recognized for thousands of years, as evinced through various artifacts of ancient cultures, evidence of this disease has been found…

GURU ANGAD DEV VETERINARY AND ANIMAL SCIENCES UNIVERSITYSynopsis

GURU ANGAD DEV VETERINARY AND ANIMAL SCIENCES UNIVERSITY Synopsis of Thesis Problem of Postgraduate Students: M.Sc Name of Student: Paramvir Singh Admission No.: L-2018-BT-05-M Major Subject: Biotechnology Minor Subject: Veterinary Microbiology Major Advisor: Dr. C.S. Mukhopadhyay 1. TITLE OF RESEARCH PROBLEM: Associating the tag-SNPs with Newcastle disease susceptibility in divergent breeds of chicken 2. INTRODUCTION…

1 CESIUM CHLORIDE CENTRIFUGATION CSCL 1974V Glisin etal discussed

1) CESIUM CHLORIDE CENTRIFUGATION (CSCL) 1974: V. Glisin et.al discussed CsCl method for purification of RNA in 1974. In this cells were first washed and homogenize then CsCl was added in cellulose nitrate centrifugation tube which act as cushion. Form top layer by adding buffer, tube were then centrifuge at 35000rpm for 12hours at 25oC….

From a very young age we’ve been told that a monetary value

From a very young age, we’ve been told that a monetary value cannot be placed on our lives – my parents would not accept any sum of money, no matter how large, in return for my life. If I happen to have children though, I’m not sure if I’ll be able to tell them the…

Poultry industry has assumed an imperative role in meeting

Poultry industry has assumed an imperative role in meeting the deficiency of animal protein in world, the Poultry production particularly broiler production is the quickest method to increase the accessibility of high quality protein for human utilization [1]. The Poultry production contrasts from animal generation in a different ways. Broiler chicken generation requires between fifty…

In order to know the gender of an individual using STR analysis

In order to know the gender of an individual using STR analysis, there is a locus on sex chromosomes called amelogenin having a region containing six base pairs longer in males than females. PCR amplification of female samples will show one band on agarose gel for amelogenin since they have two X chromosomes, while males…

xx

Over the recent years besides the traditional ECL systems such as luminol-H2O2 and Ru(bpy)32+- tripropylamine, the new ECL luminophores based on nanomaterials including CdSe, CuS, MoS2-CdS, CeO2 and CdS have been used to developing sensitive biosensors. Accordingly, yang and co-workers developed a signal-switchable electrogenerated chemiluminescence aptasensor based on ferrocene-graphene sheets (Fc-GNs) for high-efficiency quenching of…

Module 4 report Tim Johnson

Module 4: Cell Signaling Figures: Figure 1: Unknown A-type yeast cells observed for 60 minutes un constant conditions. Yeast cells were centrifuged at 1000rcf for 3 minutes, the media was then used. Unknown sample 1 was added to the media, and then cells were observed via microscope with 400x magnification. Blue represents budding yeast, grey…

The coconut palm Cocos nucifera L is a versatile important

The coconut palm (Cocos nucifera L.) is a versatile important crop cultivated in more than 90 countries in the world, with Guyana ranking as number 24 in worldwide production. Coconut is best known as the “tree of life” for its important role and contribution to the livelihoods of small farmers as a direct source of…

INTRODUCTIONDatabases could incorporate billions of bytes of

INTRODUCTION Databases could incorporate billions of bytes of data; therefore it’s ineludible that some errors can encroach in. However, the researchers UN agency work with databases would really like to stay the errors to a minimum. Error interference and correction should be essential elements of building and maintaining databases. The reasons to reduce errors area…

IntroductionDucks are a widespread poultry species found worldwide

Introduction Ducks are a widespread poultry species found worldwide. A great interest has been paid for maximizing duck’s egg and meat production in Egypt, because these birds are easily domesticated, disease resistant and also having highly feed conversion ratio (Galal et al., 2011). “In Egypt there are lots of duck breeds for example there are…

Identification Of Macromolecules Lab Report

I no color change I I Red light blue Proteins are the basis of the protoplasm (fluid living content of the cell that contains the cytoplasm and cell nucleus) and are found in all living organisms. Proteins make up the bulk of animals body’s non-skeletal structure. As enzymes, they catalyst biochemical reactions; as antibodies, they…

Restriction Enzyme Lab Report

It is thought that, together with enzymes that methyl portions of native DNA, restriction enzymes protect cells from DNA of invading organisms cutting such DNA into pieces, thereby restricting its activity. In this experiment, using agrees gel electrophoresis, the number and relative positions of restriction sites for three restriction enzymes, Score, Hinkle and Pull, on…

Sordaria Lab Report

The lab experiment examined meiosis and genetic diversity through the model organism, Sardinia familial. Meiosis is part of the sexual life cycle and occurs in all sexually reproducing organisms. It is a method of cell division that produces gametes. Meiosis has two parts: meiosis I and meiosis II. Both parts undergo the same four stages…

Unknown Bacteria Lab Report

The two streak plates made from the mixture of unknown bacteria showed two different colony morphologies. Colonies of Bacteria A were translucent in color, circular in form, with entire margins and a smooth surface. Colonies of Bacteria B were cream colored, irregular in form, with undulate margins and an ambulate surface. Two different morphologies allowed…

Gel Electrophoresis Lab Report

To examine DNA and RNA, the fragments are placed in the garage wells and an electrical charge is sent through, pushing the negatively chi argued molecules towards the positive side. The smaller the molecule, the less re’s stance it will face when hitting the pores of the gel, and the farther it will travel. Restriction…

Betacyanin Leakage Through Membranes

Damage of Beet Cell Membranes Caused by Different Temperatures Resulting in Betacyanin Leakage By: Trevor Sandum, Lab 2, March 9, 2013 Introduction: A red pigment called Betacyanin located in the vacuole of the beet cells is released when the membrane is damaged (Danyk, 2013). The membrane is necessary for sustainability of the cell’s life, it…

IDENTIFICATION OF THE DRUG TARGETMedicinal chemistry is the study

IDENTIFICATION OF THE DRUG TARGET: Medicinal chemistry is the study of how novel drugs can be designed and developed. Identification of drug target helps immeasurably by a detailed understanding of the structure & function of the molecular targets that are present in body. Major drug targets are normally large molecules (macromolecules) like proteins & nucleic…

INTRODUCTION Under normal physiological conditions the growth

INTRODUCTION: Under normal physiological conditions, the growth and division of cells occur in a controlled manner. Occasionally, cells tend to grow uncontrollably and do not respond to any growth control mechanisms. Cells which grow uncontrollably produce a clone which leads to formation of a tumour or neoplasm. Normal cells undergo molecular, biochemical, and cellular changes…

ghout the world Zhang and Li 2014 In 2013 there was 155

ghout the world (Zhang and Li, 2014). In 2013, there was 155 million affected by CIS globally (Feigin et al., 2016). In Egypt, the incidence of stroke is high with a crude prevalence rate of 963/100,000 (Abd-Allh and Moustafa, 2014). The CIS may have considered a vascular disease (Tian et al., 2018), dyslipidemia is considered…

Describe how a gene encoding a therapeutic protein could be

Describe how a gene encoding a therapeutic protein could be cloned into a vector to allow expression in gene therapy. Over 60 years ago, the scientists James Watson and Francis Crick first discovered the structure of DNA. From this discovery, other scientists have built on their knowledge to form techniques known as ‘gene therapy’. Gene…

Disease causing protein of Alzheimer’s diseaseUniversity of

Disease causing protein of Alzheimer’s diseaseUniversity of Toronto ScarboroughProfessor Shelley bruntBiochemistry I: Proteins and Enzymes: bioc12hZhi Xin Huang1003348424 Protein misfolding disease (PMD)Christian Anfinsen demonstrated that protein conformations form accordingly based by their primary amino acid sequence It is understood that mutant proteins may be more susceptible to protein misfolding (Zhang et al., 2004)However, wild-strain proteins…

MATERIALS AND METHODSStudy populationIn this crosssectional

MATERIALS AND METHODS Study population In this cross-sectional study conducted from January 2018 to march 2019, a total of 90 Iranian patients with ?-thalassemia major referred to Shafa Thalassemia Clinic were enrolled. Inclusion criteria contained confirmed cases of ?-thalassemia patients regularly receiving at least one unit of blood per month. patient?s medical files were reviewed…

Gel Electrophoresis Research Paper

Gel Electrophoresis is a technique in molecular biology and biochemistry, which is used for the separation of proteins and nucleic acids, based on the motion of charged biological macromolecules in a constant electric field. Polyacrylamide gel separation is due to differences in molecular charge and separated differences of molecular weights and the configuration of molecules….

Analytical Device

A biosensor is an analytical device which uses biologically-sensitive material to detect biological or chemical species directly without the need for complex sample processing. It is usually made by attaching a biologically-sensitive material to a suitable transducing system, which converts the biochemical response into a quantifiable and processable electrical signal (Ngweinbi & Suleiman, 1990). The…

Gfp Lab Report

All four of the wells were condensed with I Pl of an enhancer and added too lipid-based transaction reagent Effected to allow the target DNA to be introduced into the Hell cells. The cells containing . 45 ml Of transaction complexes and complete growth medium were incubated for two days and then replaced With I…

Gmo Lab Report

At this rate, Genetically Modified Organisms within food products will increase dramatically, but is this for the best? There are many sis factors that have been brought up with genetically modified foods such as potential increase of cancer according to a French study conducted by Dry. Giles Eric Israelis (Kalmia, 2012). Genetically modified food is…

Essay Assay

Enzyme-Linked Immunosorbent Assay ( abbreviated as ELISA ) , is a sort of immunochemical assay technique to observe the presence of a specific antibody or antigen in the trial samples. This method chiefly make usage of both immunological reaction ( the antibody-antigen reaction ) to observe the nowadays of specific antibody or antigen ) and…

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