Enzyme Activity Prediction for Sucrose at 60°C

Enzyme Activity Name: Daniel Franco Instructor: Professor Jennifer Freer Date: 0308. 2015 Predictions Sucrose will have the greatest activity at 60 ?co (140 Sucrose activity decreases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature. Amount of substrate (sucrose) present, sucrose 4 sucrose incubation time

Effect of Temperature on Enzyme Activity temperature pH, amount of substrate (sucrose) present, sucrose + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity amount Of substrate (sucrose) present temperature, pH, sucrose + sucrose incubation time 4.

Describe what is measured as an indicator of sucrose activity and why this is an indicator Of sucrose activity. Optimal density is measured because it measures the amount of glucose and fructose produced. 5. Explain why denatured sucrose was used as a control.

Denatured sucrose was used as the control because activated sucrose was used to increase the activity. Laboratory Report/ Daniel Franco/ Enzyme Activity/ Professor Jennifer Freer/ 03.

08. 2015/ Page [1] of [4] 6, Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrose. Explain why it is important to denature sucrose before measuring product concentration. In order to decrease enzyme activity.

Franco Lab