Diagnosis Of Cervical Cancer

The HeLa cell line was formed from Henrietta Lacks, a thirty-one-year-old African American who was diagnosed with cervical cancer. Howard Jones, a gynecologist, informed Henrietta that her biopsy came back positive for cervical cancer. When Henrietta went for her first treatment, Dr.Wharton Jr scraped two pieces of tissues from Henrietta’s cervix, without informed consent from her. He passed the tissue to Dr. George Gey, a researcher at John Hopkins hospital. Henrietta’s cells weren’t like the other patients who cells just died.

Her cells were growing instantly in the medium culture and doubled every twenty-fours hours. George Gey created the HeLa cell line. HeLa came from the first two letters of her first name, Henrietta and last two letters of her last name, Lacks. The HeLa cell line was significant in the history of biomedical research because it helped developed drugs for herpes, leukemia, Parkinson’s disease; along with the creation of the polio vaccine, cloning, gene mapping, and the studies of HIV/AIDS, lactose digestion etc.

The hypothesis being tested is in this research is a-mangostin(AM), an organic compound used towards the apoptotic effect on HeLa cells. The cytotoxic effects of the organic compound, a-mangosteen on the growth and development of HeLa cells and a human ovarian cell were analyzed by using MTT assay. MTT is an essay that measures cell metabolic activity. The results showed that a-mangosteen repressed HeLa cells growth and development based on concentration and time-dependent as a factor. The HeLa cells had a IC50 value of 24.

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53± 1.48 at twenty-hour hours. To find these results, the effects of a-mangosteen on HeLa cells were evaluated by using fluorescence microscopy analysis. This experiment showed that a-mangostin could be a potential factor in cervical cancer in vitro.

Yes, I think Henrietta’s family should be financially compensation because the doctor took cells from her cervix without formal consent or permission. Two, not only did he not get permission, he gave Henrietta’s cells to other researchers at laboratories and they passed HeLa cells on to the point where Henrietta’s cells were being sold for money and cloned. I don’t think that it’s fair to the Lacks family because while their mother’s cells are helping people out around the world and being used in experiments, if the experiment is effective, the scientist or creater is getting rewarded and not the family. Another reason why I think Henrietta’s family should get compensation because back in the 1920’s, they lived in poverty and didn’t have the money to be financially stable, so with the money for the cells would help the whole entire family out. If I owned a biotechnology firm that uses HeLa cell lines, the first thing I would do is contact the Lacks family and have them come and look at their mother’s cells.

Also, if they wanted to take their mother’s HeLa cells home, they have my permission. If I was a relative of Henrietta Lacks, I would get official documentation for the biotechnology firm to give me the original copies of my mother’s cells.  The human subjects in this research is SCA (spinocerebellar ataxia) patients and normal human subjects. There are 17 SCA patients total; eleven with spinocerebellar ataxia type 6 and six with spinocerebellar ataxia type 31. There were also seventeen normal subjects. The findings in this paper showed that the spinocerebellar ataxia (SCA) patients were relatively slower to tap the button on a keyboard than the normal human subjects. The results of the data was SCA patients: had 379.3±114.ms while the normal subjects had: 266.7±66.3 ms. This could mean that the patients has a non-motor cerebellar dysfunction. Yes, informed consent was discussed in the paper. The statement was that all subjects who participated in this study was written informed consent.

References

1. Cited Aisha I. El habbash, et al. “In Vitro Assessment of Anti-Proliferative Effect Induced by Α-Mangostin from Cratoxylum Arborescens on Hela Cells.”
2. Peerj, vol. 5, 2017, p. 3460. doi:10.7717/peerj.3460.
3. Matsuda S, Matsumoto H, Furubayashi T, Hanajima R, Tsuji S, Ugawa Y, et al.
4. “The 3-Second Rule in Hereditary Pure Cerebellar Ataxia: A Synchronized Tapping Study. PLoS ONE vol. 10, 2015, p. 14 doi:10.1371/journal.pone.0118592. Skloot, Rebecca.
5. The Immortal Life of Henrietta Lacks. Broadway Paperbacks, an Imprint of the Crown Publishing Group, a Division of Random House, 2011.