My Lab partner And I Had The

Unknown bacteria number seven, we have come to the conclusion that this is Pseudomonas aeruginosa. When we conducted out morphology testing, we found a small pink colored rod, which entailed that it was gram negative. We found our specific strain to be non-motile even though it is a motile bacterium. When we grew the pseudomonas on a tryptic soy agar it grew green, this specific color change is what helped us initially come to the conclusion that this was our unknown bacterium.

Pseudomonas is an aerobic bacterium that grows in an optimal temperature of 420 C. Pseudomonas will grow in the absence of O2 if in the presence of NO3 because it is a respiratory electron acceptor to pseudomonas. We observed our bacterium on our agar plates and determined that they were abundant in growth with a wrinkled configuration and a wavy margin.

We believe that we have correctly identified our unknown bacterium because when we did all our testing It pointed to Pseudomonas aeruginosa.

However, we did get some false negative results that threw us for a loop. When conducting our test initially looking at motility we came up as negative when pseudomonas is supposed to have a positive motility, this threw us off for the first week because we thought that we had a bacterium with negative motility, when we continued further testing we got another false positive when conducting the oxidase test were suppose to get a black swab indicating that it was positive for oxidase, however our swab did not change color after the proper amount of time allotted indicating that our bacterium was not producing oxidase.

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We were correctly able to identify our bacterium because not only is ours unique in the fact that when it was growing on our tryptic soy agar plate it was growing green, but we also got the rest of our results to match. Pseudomonas was also the only bacterium in our particular lab that has the ability to break down animal protein so when we got our test results back and ours was the only one that liquified the gelatin it further helped solidify our identification. Pseudomonas aeruginosa was first discovered in 1882 by Carle Gessard a French pharmacist. He discovered that P.Aeruginosa was a water soluble pigment which glowed blue green under the exposure of ultra violet light.

“Pseudomonas aeruginosa is notorious for its resistance to antibiotics and is therefore a particularly dangerous and dreaded pathogen.” It is resistance to many antibiotics because it has a permeability barrier. It has a tendency to colonize surfaces in a biofilm form which makes the cells impenetrable to concentrated antimicrobials. Pseudomonas is known for causing Respiratory infections, septicemia and ear infections. It is also known for UTI or urinary tract infections as well as infections in the gastrointestinal tract. It is infections in nature and is considered a potential super bug. With the potential for It to be used as a biological weapon its genome is constantly updated and monitored in all of the CDC and other governments databases.

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