The proper name for Blackmore is Luminous Variegates, they are freshwater worms most commonly found in North America ND Europe. The blood in Blackmore circulates from the dorsal blood vessel toward the head through a series of rhythmic contractions. A common pulse rate for the Blackmore is 7 to 8 pulse/minute. Hypothesis. If a stimulant increases heart rate, then when the Luminous Variegates is put into Nicotine its heart rate will increase. Materials and Methods: -First, fill a Petri dish with water and insert 15 blackthorns into the dish from the bucket containing all tot the Blackmore.
Extract one worm into a pipette then insert into the capillary tube, Place the capillary tube under a microscope and adjust until the blood flow is seen. Pick one segment and count how many times it contracts during a minute’s time, use the pipette and push the worm out of the capillary tube and into another Petri dish for the Petri dish which contains the worms that have already had their control pulse rate counted), Continue these steps doing one control and one nicotine test for each worm and make sure to count pulse rates from the same area of each worm that the data is as consistent as possible.
Next, get another Petri dish and fill with 2. 5 ml of unfiltered non-menthol Nicotine along with I ml H2O. Take one form from the “trash” dish and insert into the Nicotine for one minute, make sure to measure how much water is getting into the Nicotine to know how much the solution is diluting.
This was controlled so that we could make sure each worm was being put into the same strength nicotine solution. Take the worm and put it into a separate pipette and then another capillary tube and measure the pulse rate for one minute.
Take the worm out and put it into the “rehab” Petri dish, a place for the worms to get their heart rates back to normal before joining all of the worms. Continue these steps and record the pulse rate once in the nicotine for each of the worms. Make sure to calculate both the mean and median from both the control and stimulant trials. Results: -The dependent variable is the pulse rate of the Blackmore in pulse/min. The independent variable is the nicotine added which is 2. Ml with ml H2O.
The same IS worms were used in both trials ( each worm was tested once for each), the same Nicotine was used tort all stimulant trials, worms were in the Nicotine for the same amount of time, the pulse rate was recorded at the same time for ACH worm, same capillary tubes were used, same pipette was used, and worms were put into the same Petri dishes. Two trials were done with the Nicotine at ml and nine trials were done with 2. 5 ml Nicotine along with 1 ml H2O We had to dilute the solution because every worm in the 2. Ml Nicotine solution died. Spring water served as the positive control since it serves as the worm’s everyday habitat.