We have made four set-ups in two different specimens. Onion and Boat of Moses skin with water, 2%, 5% and 10% salt (NCAA) Solution. Based on our experiment, the result of the set up explains that the presence of the salt solution results in outward diffusion of water and the collapse of the protoplasm. In table 1, we have observed under the microscope that the onionskin mounted with water has no results of polymerase cell (0%). This time, we have repeated the procedure with 2% salt solution and it is observed that there are 6 (3. 9%) localized cells. In the 5% salt solution, there are 119 (87. %) polymerase cells. And in the 10% salt solution, there are number of polymerase cells present. In table 2,same procedure was done but with different specimen. We have observed that the boat of Moses mounted with water also has no results of polymerase cell (0%). In the 2% salt solution, there are about 20 (43. 4%) polymerase cells. 46 (88. 5%). Polymerase cells were also observed in the set-up with 5% salt solution and in the solution, all of the 59 cells are polymerase (100%). Thus, increase in percentage of the salt in a solution also increases the number of the polymerase cells.
Water passes out of the cell towards a region of lesser water concentration which results to the shrinkage of the protoplasm (Figure 1). This shrinkage away from the cell wall due to excessive loss of water is called polynomials. Figure 1. Polynomials in Ilium Cheap : The first image in the left shows that the cells mounted with water shows no signs of polynomials, in the middle mounted with 2% NCAA solution shows minimal shrinkage of cells, in the right most image mounted with 5% NCAA solution almost all of the cells were polymerase.
After observing the onion and boat of Moses skin in the different solution, we have replaced the solution with distilled water. Based on our observation, when the solution is replaced, the protoplasm regained to its original form simply because of the vacuole that absorbs and stores the water. This then results to the inward diffusion of water. “If after a short time, however, the polymerase cells are transferred from the salt solution to pure water, the direction of the et water movement is reversed, for there is now relatively more water outside the cells than there is inside.
Water thus passes out inward, the protoplasm increases in volume as the outdoor pressure of the cell sap increases, and the tissue regains its turgidity. ” (H. Fuller and Z. Caruthers) Reference: The plant world fourth edition Harry Fuller and Zane Caruthers 1963 United states of America: Holt, Rinehart and Winston, Inc.