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Factors Effecting Action of Lipase Essay

Effect of Temperature on the action of LipaseAimThe aim of this experiment is to find out how the temperature effects the action of lipase.Method* A glass beaker was taken and filled with about halfway with water.* The water was then heated to 50�c* Into a boiling tube 5 cm� of milk, 7 cm� of sodium carbonate and a few drops of Phenothalin were placed.* The boiling tube was placed into the water for a few minutes so it could warm to the temperature of the water.* Then 1 cm� of lipase was added and the stopwatch started.* When the reaction had finished (i.e. the milk wasn’t pink anymore) the stopwatch was stopped and the time recorded.* Other groups did different temperatures and all the results were shared.Apparatus* Boiling tube* Milk* Sodium carbonate* Lipase (5% conc.)* Phenothalin* Glass beaker* Tripod* Gauze* Bunsen burner* Heat proof mat* Thermometer* Stop watchPredictionI think that the 30�c experiment will have the fasted reaction rate because it is the closest to body temperature where the enzymes naturally work. At 100�c I predict that there will be no reaction because the enzymes will be de-natured because it is so hot. And at 0�c I predict there will also be no reaction as it will be too cold for the enzymes to work.Variables* Amount of milk – 5 cm�* Amount of sodium Carbonate – 7 cm�* Amount of lipase – 1 cm�* Heat – 0�c, 13�c, 30�c, 50�c, 100�cResultsTemperature (�c)1st time (s)2nd time (s)Average (s)0(No result)(No result)N/A13671757714302282513535095111103100(No result)(No result)N/AAnalysisThe results and the graph clearly show that at 0�c and at 100�c a reaction did no take place. At 100�c the lipase was denatured but at 0�c it was just too cold for it to react quickly enough for it too be seen. The results also show that at 50�c the reaction is the quickest and so therefore proved me wrong, as I believed that 30�c would be the temperature that the lipase reacted quickest at, but it wasn’t.The experiment went well and some good results came from it. It would be interesting to try more different temperatures and so then a better more accurate graph would be achieved, it would also be interesting to find out the point at which the lipase is actually denatured (i.e. the highest temperature that the lipase can work at). And if I were to do further work on these experiments I would most definitely choose to go down that line of experiments. It would create some interesting results.

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