A spectrophotometer will be used to measure this variable by measuring the absorbency value of the beautician in solution after each treatment. The results of this study will show the necessary temperature to keep the vacuole intact. It would influence common agriculture practices by indicating a more ideal environment setting and techniques. At the same time, it would allow scientists to have a better understanding of cell structure and functions. We hypothesize that beet cells in a high temperature environment will release more beautician in comparison to beet cells in a room temperature environment.
The increased temperature will increase the damage of membranes, thus no longer able to enclose beautician within the vacuole. In contrast, as temperature decreases, the amount of beautician will also decrease due to the less damage of the membrane. Materials and methods Seven discs of mm beet root were cut with a razor blade and rinsed with tap water in a plastic beaker for 10 minutes. Each beet disc was then submersed in a eager of water with temperature of either -ICC, ICC, ICC, ICC, ICC and room temperature for precisely 2 minutes.
The exact time of extraction was recorded when the discs were placed into large test tube with white caps (labeled with temperatures) immediately after the water bath. The test tubes were then rested on the bench for a total of 10 minutes and were vortexes at 5 minutes and 10 minutes. After 10 minutes, 5 ml of solution from the large test tubes was pipettes into small test tubes and each small test tube was then inserted into the phosphorescent for measurements of absorbency.
A detailed protocol can be found in the Biology 107 Laboratory Manual (2010-2011).
Results In our experiment, we observed that the samples in temperature -ICC and ICC contained the highest concentration of beautician (Figure 2). To obtain these results, we used the standard curve for beautician (Figure 1) to figure out the concentration, since the value of beautician absorbency could be measured using the spectrophotometer. From the graph (Figure 2), between the temperature of ICC, ICC, ICC, it was observed that the ranges of concentration as narrow, which shows the increase concentration was quite steady.
Generally, an increase of temperature would cause an increase in absorbency of beautician, thus an increase of concentration. However, it was also observed that at -ICC and ICC, there was a sudden jump of data (Table 2-2). Originally, the absorbency measured for samples in -ICC and ICC were 1. 700 and 0. 845; the concentrations were 425 pm and 180 pm respectively. However, because both of these absorbency readings were greater than the highest absorbency value in our standard curve (Figure 1), a calculation of diluted concentration for these two samples was necessary.
Therefore, using the formula Cue = Cd/D where Cue = concentration of undiluted (original) sample, Cd = concentration of diluted sample and D = dilution (which is 1/5 in this experiment), we calculated that the diluted concentration for the sample in ICC is 85 pm and for the sample in ICC is 36 pm. As we inserted these data on the graph (Figure 2), concentration of beautician for samples in -ICC and ICC still dominate in comparison to others. This sudden increase of concentration allowed us to understand that the topmasts membrane is easier to be damage in extreme temperature.
Discussion Our original hypothesis about the effect of high temperature on beet cell membrane was correct; as temperature increases, the concentration of beautician increases as well. The beet root sample in temperature ICC had a higher absorbency value and concentration of beautician in comparison to other samples due to the membrane damage. The experiment of lipid membranes performed by the University of Osaka concluded a similar result. They found that the membranes were stable at physiological temperatures, but there were release of DOC from lollipops after heating, which indicates that the membranes have been damaged (Keno et al. 2010). However, our data showed that the sample in -ICC had an even higher absorbency value and concentration of beautician than the sample in ICC. Therefore, we concluded that temperature -ICC caused even more damage on membrane than high temperature ICC. Either way, both of these data suggested in order for membrane to keep colloidal stability and experience minimal damage, imperative of environment must not be extreme (Keno et al. , 2010).
Otherwise the structure of the biological molecule will be disrupted, leading to malfunction of osmotic balance and improper functioning of the cell (Campbell et al, 2008). We began our experiment with individual dilution and procedure for each temperature treatment, which took a long period of time. Therefore we had to start performing 2 different temperature treatments at the same time. This may have caused some inaccuracy in extraction times and the time intervals in which solutions needed to be vortex. We also spilled some of the solution while attempting to transfer the solutions to the small test tubes.
This may have caused some variations in the range of some of the measurements. In order to improve and be able to acquire more accurate results next time, we will ensure there is a sufficient amount of time for each temperature treatment. We will also ensure accurate extraction times and time intervals for overexerting solutions, as well as avoiding any solution spillage. This experimental design was valid. However, in order to improve and be able to acquire more detailed results here number of manipulating variable should increase.