Essays on Chromatography

An Internship in Indian Institute of Science Education and Research
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Since, I was a child chemical reactions and the magical colors they produced fascinated me to know the actual reason behind the process. When in the 9th grade I was introduced to the subject of chemistry, I was instantly attracted towards it and its contribution for the benefit of mankind in the field of pharmaceuticals, fertilisers etc. My interest towards chemistry from 11th class onwards gradually increased manifold as I gathered deeper knowledge of the subject. As the syllabus increased,…...
High Performance Liquid Chromatography
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Introduction Anhydrous caffeine is found as a white crystalline powder or white crystals, in water it is sparingly soluble but becomes freely soluble when the water is boiling (, 2019). Caffeine is an alkaloid xanthine derivative (Tarnopolsky, 2010) that acts as a psychostimulant and mild diuretic to humans and animals and is found within the beans, leaves and fruit of more than 60 plants, (Institute of Medicine (U. S.) et al., 2014), it is mostly consumed by humans as coffee…...
Methods for detection of sulfites
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Literature review There are many methods for detection of sulfites and Its important to asses one method and know its importance. Two liquid chromatographic frameworks were utilized. Although the two frameworks had an UV6000 diode cluster locator with a 50 mm light way and an AS 3000 auto sampler. Moreover the framework with the Fast Acid section utilized a Dionex GPM-2 slope siphon at a stream rate of 1.0 mL/min. Additionally the framework with the HPX-87H section utilized a ThermoQuest…...
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Ldh Purification Lab Report
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This sample essay on Ldh Purification Lab Report provides important aspects of the issue and arguments for and against as well as the needed facts. Read on this essay's introduction, body paragraphs, and conclusion.OLD was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometers determination of NADIA at 340 NM. From Pierce BCC assay of crude homogenate, initial protein concentration was shown to be 100 MGM/ml. The final protein concentration of…...
Pigments In Spinach
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This essay sample on Pigments In Spinach provides all necessary basic information on this matter, including the most common "for and against" arguments. Below are the introduction, body and conclusion parts of this essay.Investigation of Photosynthetic Pigments in Spinach Michael Yacoub BIOL&211 Lori Hayes Introduction: Purpose: To demonstrate the existence of the pigments of photosynthesis in spinach by separating them using chromatography, then measure them using spectrometry. There are multiple pigments in photosynthesis, the varying pigments allow the plant to…...
Tlc Lab Report
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The essay sample on Tlc Lab Report dwells on its problems, providing a shortened but comprehensive overview of basic facts and arguments related to it. To read the essay, scroll down.Food color additives are dyes, pigments or substances that impart color when applied to a food, drug, cosmetic, or the human body. The Food and Drug Administration (FDA) is responsible for regulating all color additives used in the United States. All color additives permitted for use in foods are classified…...
Kool Aid Chromatography
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When working in a lab, scientists often need to identify different molecules that are present in a sample they are studying. There are many ways to identify unknown molecules/chemicals in a sample. The method you will be using today is called Paper Chromatography and consists of 2 steps. First, you will separate the unknown chemicals and then you will identify them. This process can also be applied to the food we eat everyday.When you eat food or drink liquids you…...
Chromatography Lab Report
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Reece’s yellow, Reece’s orange, Reece’s brown, pink pen and green pen all appear to contain only one component. . Which colors were consistently composed of more than one color? 1 5 points) – Green food coloring and blue food coloring appeared to be the only ones consistently containing more than one color. 7. Why does the technique require you to use a pencil to mark the original spotting line? (15 points) – The pencil is used to mark the original…...
ChemistryChromatographyColorForensic ScienceScientific Method
Gas Chromatography Lab Report Essays
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Gas chromatography separates the component of a sample based on the ease with which they evaporate into a gas, also known as the volatility. The chemical mixture being analyzed is injected into the injection point and carried through the instrument by an inert gas, in this case Helium. The temperature of the injection point is approximately ICC and the oven temperature is around ICC. There is a column in the oven that makes it Seibel to separate the chemicals based…...
ChemistryChromatographyManufacturingMaterialsMechanical EngineeringScientific Method
Salt And Sand Separation Lab Report
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Evaporation is a process where two liquids or solid mixed into a liquid can be separated. To separate two liquids mixed together, the liquid is boiled over a Bunsen burner until one liquid is completely evaporated. To separate a solid within a liquid, the liquid is completely evaporated and a solid will remain, which is called a residue. Procedure/Data: 1. Preheat a clean evaporating dish for 5 minutes; then cool and mass the dish. Mass of empty dish: 28. Egg…...
Thin Layer Chromatography Lab Report
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Chromatography works on the principle that different impounds will have different solubility and adsorption to the two different phases between which are to be partitioned. Thin Layer Chromatography (TTL) is a solid-liquid technique. The mixture is observed when it is in two different phase; a solid (stationary phase) and a liquid (moving phase). Thin Layer Chromatography (TTL) is a technique which is sensitive, cheap and fast. A thin layer chromatograph plate is used to identify drug component under LIVE. It…...
METHODOLOGY31 Collection of water samples
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METHODOLOGY3.1 Collection of water samplesBased on the contracted laboratory’s request, two bottles of one-liter water samples were collected from the three chosen buildings of the study: Roque Rua?o 3rd floor drinking fountain (near Computer Laboratory), Albertus Magnus 2nd floor drinking fountain (near Food & Technology Laboratory) and the Main Building 1st floor drinking fountain (near Science Computer Laboratory). Based on WHO’s guidelines for drinking water quality, water was collected in polyethylene bottles at a low temperature of less than or…...
Calcium CarbonateChemistryChromatographyMaterialsNaturePh
B5 Research Approach and Methodology B5A Statement of
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B.5. Research Approach and MethodologyB.5.A Statement of Proposed ResearchApproach:The idea of the approach is to synthesize a topical ointment that the lawsone encapsulated into a nano-emulsion particle will be the active ingredient. The produced ointment will then be tested in viro on Hacat cell lines and in vivo on mice models suffering from the infection. This ointment is expected to produce a bactericidal effect on staphylococcus bacteria which causes the cellulitis skin infection. In vitro testing will be done on…...
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BUFFER EXCHANGEThe PD10 column is prepacked with Sephadex G-25 which works in the principle of size exclusion chromatography. The pores of Sephadex beads allow small molecular weight substances to pass through and prevents high molecular weight one from entering into the pores. Hence, the order of elution is first high molecular weight followed by low molecular weight as low molecular weight substances will take longer time to move. In this case, PD-10 column is used to transfer the protein to…...
BiologyChemistryChromatographyScientific Method
Post Lab Report
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This process aids in determining affinity. 3 The most polar compound is always the absorbent; however, the polarity of the compounds in the mixture in addition to the solvents found in the mixture differs in polarities. We use chromatography, or more specifically TTL, to determine the relative amounts of components in a mixture. Additionally TTL is utilized to determine the identity of two substances. When utilizing TTL, the ratio of distance traveled by the compound to the solvent is defined…...
ChemistryChromatographyExperimentScientific Method
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